The Cerrado is the world's largest savannah and one of Brazil's richest biomes in terms of biodiversity. Its plants have fruits with high nutritional potential. Despite being rich in nutrients, fruits from the Cerrado are seldom consumed due to a need for more awareness about their nutritional value and challenges in marketing. Thus, this work aims to investigate the nutritional composition of the fruits of the Brazilian Cerrado: buriti (Mauritia flexuosa), pequi (Caryocar brasiliense), murici (Byrsonima crassifolia), guabiraba (Campomanesia xanthocarpa), tucum (Astrocaryum vulgare) and tuturuba (Pouteria macrophylla). Buriti is a significant source of carotenoids, but these compounds have been extracted using laborious and low-effective methods. Thus, buriti was used to optimize the ultrasound-assisted extraction (UAE) method of carotenoids from the pulp. First, the extraction solution was optimized, with the highest yield for the acetone:ethanol mixture (75/25), then mass (80 mg) and time (30 min). For minerals, murici and buriti stand out, with the highest concentrations of Ca (69.1 and 56.0 mg/100 g), Fe is more abundant in tuturubá (29.6 mg/100 g). Bioaccessibility, performed in vitro, showed an average range of variation from 28.4 to 37.7% (K and Ca) concerning the total mineral content, where the global bioaccessible average was 33.9% of the total concentration. The dialyzable fraction (bioavailable concentration) showed a more irregular behavior, with average percentage contents ranging from 7.6 to 20.3% (Mg and Ca). About phenolic compounds, the highest concentration was found in tucum and murici (1640.8 and 1439.3 mg GAE/100 g), but pequi and guabiraba had the lowest (410.9 and 467.5 mg GAE/100 g). Buriti was the one with the highest concentration of carotenoids. Tuturubá, whose carotenoid content was not yet known, presented a content of 204 ± 13 µg g-1, close to that of pequi (211 ± 9 µg g-1), more widely studied. In addition, tuturubá and pequi are good protein sources (139.7 and 124.7 mg g-1) and fiber (38.7 and 33.4%). The highest percentages of lipids were observed for pequi and buriti (60.6 and 50.4%). Principal Component Analysis was performed to confirm whether any sample had a degree of similarity. Total, accessible, and dialyzable potassium, carotenoids, and fiber concentrations were the variables that most influenced PC1 (38%). These variables differentiate the pequi and buriti samples from the others studied. These results highlight the importance of Cerrado fruits as valuable sources of nutrients, which suggests their potential as functional ingredients and health promoters. Valuing and encouraging the consumption of these native fruits can contribute to diversifying the diet and preserving the biodiversity of the Cerrado.